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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
05/01/2001 |
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Data da última atualização: |
05/01/2001 |
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Autoria: |
RITCHIE, N. J.; SCHUTTER, M. E.; DICK, R. P.; MYROLD, D. D. |
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Título: |
Use of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 66, p. 1668-1675, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
In length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method is an efficient, reliable, and highly reproducible method that should be a useful tool in future assessments of microbial community composition. MenosIn length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02152naa a2200157 a 4500
001 1621188
005 2001-01-05
008 2000 bl --- 0-- u #d
100 1 $aRITCHIE, N. J.
245 $aUse of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil.
260 $c2000
520 $aIn length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method is an efficient, reliable, and highly reproducible method that should be a useful tool in future assessments of microbial community composition.
700 1 $aSCHUTTER, M. E.
700 1 $aDICK, R. P.
700 1 $aMYROLD, D. D.
773 $tApplied and Environmental Microbiology, Washington$gv. 66, p. 1668-1675, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
08/11/2011 |
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Data da última atualização: |
08/11/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GALISA, P. S.; ARAUJO, J. L. S. de; REIS, V. M.; VIDAL, M. S. |
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Afiliação: |
PÉRICLES DE SOUZA GALISA, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; VERONICA MASSENA REIS, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
Avaliação da expressão gênica em raízes de cana-de-açúcar inoculada com bactérias diazotrófcas através da técnica de microarranjo. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia, 2011. |
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Páginas: |
37 p. |
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Idioma: |
Português |
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Palavras-Chave: |
Cana-de-açúcar; Fixação biológica de nitrogênio; Microarranjo. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/45798/1/AVALIACAO-DA-EXPRESSAO.pdf
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Marc: |
LEADER 00760nam a2200181 a 4500
001 1905243
005 2011-11-08
008 2011 bl uuuu u00u1 u #d
100 1 $aGALISA, P. S.
245 $aAvaliação da expressão gênica em raízes de cana-de-açúcar inoculada com bactérias diazotrófcas através da técnica de microarranjo.$h[electronic resource]
260 $aSEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia$c2011
300 $a37 p.
653 $aCana-de-açúcar
653 $aFixação biológica de nitrogênio
653 $aMicroarranjo
700 1 $aARAUJO, J. L. S. de
700 1 $aREIS, V. M.
700 1 $aVIDAL, M. S.
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